Two specimens must be submitted; one in formalin and one in Zeus media tissue fixative.

Formalin vial

Zeus media tissue fixative

Pathology Tissue Exam

1. After excision, place one specimen into a container containing 10% neutral buffered formalin.

Note: The specimen should be placed immediately in 10% neutral buffered formalin unless a frozen specimen is needed

2.  Properly label specimen container and completely fill out the request form or answer all Excellian order questions.

Immunofluorescence (IMF)

1. After excision, place second specimen into Zeus media tissue fixative.

2.  Properly label specimen container and completely fill out the request form or answer all Excellian order questions.  Must request IMF in the description field for Excellian orders or by writen request on a manual/paper requisition.  

Requisition requirements

Manual request forms (all) or Excellian Supplemental Form (FNA, Breast and Path Consult only)

• Label with two unique identifiers:  Patient’s full name and date of birth
• List the specimen source and anatomic site (i.e. Mole, Left forearm)
• Use A, B, C, etc. with multiple specimens from the same patient
• Excellian Sites: Order and collect the Path Tissue Exam test and complete all ask on order questions.

Specimen container requirements

• Label with two unique identifiers:  Patient’s full name and date of birth
• Label the jar; not the lid
• Label each jar with the letter (A, B, C) and specimen source and anatomic site to exactly match the description on the requisition
• Verify that all information on the requisition exactly matches the specimen jar labeling to prevent a delay in specimen processing.

Formalin vial and Zeus media tissue fixative

Hennepin Healthcare Immunofluorescence (IMF) requisition

Ambient (strict) - 4 days

Refrigerated - NO

Frozen - NO

• Samples containing SPS, citrate, or heparin
• Blood/Serum specimens
• Samples collected on swabs other than E-swabs

Path Tissue:  Microscopic evaluation

IMF:  Immunofluorescence (IMF)

An interpretive report will be provided