Lyme disease, line blot

Alphabetical Test listing

Lyme disease, line blot-14520

Test name:

Test number:

14520

Excellian order number:

LAB14520

Abbreviation:

LWB

Alternate names:

Borrelia burgdorferi antibodies

Tests included:

Line blot analysis and interpretations for IgG and IgM-specific antibodies

Useful for:

Detect antibodies specific for B burgdorferi.
This test is intended as the second step in testing serum samples found to have been equivocal or positive

Specimen type:

Serum

Collection container:

Gold serum separator (SST) tube

Volume:

1.0 mL

Minimum volume:

0.5 mL

Collection instructions:

Immediatley following collection, mix sample by inverting 5 times

Processing instructions:

Allow sample to clot for a minimum of 30 minutes
Spin within two (2) hours of sample collection

Transport container:

Gold serum separator (SST) tube

Alternate collection container:

Red serum vial/tube, 5 mL

Alternate collection instructions:

Allow sample to clot
Spin
Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp) within two (2) hours of sample collection

Alternate transport container:

Transfer vial/tube with cap - 12mL (LabCorp)

Transport and stability:

Ambient (preferred) - 14 days

Refrigerated - 14 days

Frozen - 14 days

Freeze/thaw cycles - stable x3

Reason for rejection:

Hemolysis
Lipemia
Gross bacterial contamination

Performing lab:

LabCorp (163600): R-LC

Days set up:

Mo - Fr

TAT:

5 days

Method:

Line blot

Reference ranges:

Negative

Clinical information:

Provides both IgM and IgG Line blot results. Increased specificity reduces cross-reactivity from other spirochetes. Detect and analyze antibodies against specific B burgdorferi proteins. The sensitivity of a Line blot assay by itself is not sufficient to assess Lyme infection adequately. Both EIA and Line blot tests should be performed.

CPT codes:

86617 x 2

Date created:

06/21/2022

Revised date:

06/13/2023

Review date:

05/30/2023