Herpes simplex virus (HSV) 1/2, PCR, blood
Herpes simplex virus (HSV) 1/2, PCR, blood-13527
HSV 1/2 PCR
This test is intended for use as an aid to the diagnosis of herpes simplex virus (HSV) infections; it also differentiates HSV-1 from HSV-2. PCR testing of blood, serum or plasma samples is clinically useful ONLY in potential cases of disseminated HSV infection (neonates, immunosuppressed individuals) and not as an aid in the diagnosis of either mucosal or CNS disease.
HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.
- The HSV 1/2 blood assay is not intended for use with cerebrospinal fluid or for prenatal screening.
Immediately following collection, mix sample thoroughly by gentle inversion, 5 times
- Allow sample to clot for a minimum of 30 minutes
- Spin within two (2) hours of sample collection
Gold serum separator (SST) tube
ACD whole blood or plasma
Red:
- Allow sample to clot
- Spin within two (2) hours of sample collection
- Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum
Lavender/Yellow:
- Spin within two (2) hours of sample collection,
- Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as the appropriate plasma type, or submit whole blood in original collection container
Whole blood:
Lavender (EDTA), 4mL or Yellow ACD (A or B)
Plasma:
Transfer vial/tube with cap - 12mL (LabCorp)
Serum or plasma
Ambient (preferred) - 7 days
Refrigerated - 7 days
Frozen - 90 days
Whole blood
Ambient (preferred) - 7 days
Refrigerated - 7 days
Frozen - NO
PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.
Real-time polymerase chain reaction (RT-PCR)
Negative