Lt blue Sodium citrate (NaCit)

Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.

• Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.
• The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood.
• A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes.
• Any tube containing an alternate anticoagulant should be collected after the blue-top tube.
• Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes.

Citrated plasma samples should be prepared by double centrifugation.

1. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells.
2. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes.
3. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube.
4. Transfer the plasma into a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)
5. Freeze immediately and maintain frozen until tested.

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Frozen - 14 days

• Freeze/thaw cycles - stable x3

• Severe hemolysis
• Improper labeling
• Clotted specimen
• Specimen diluted with I.V. fluids
• Samples thawed in transit
• Improper sample type
• Sample out of stability

Chromogenic

80 - 150 %

Decreased levels may lead to easy bruising, epistaxis, hematuria, menorrhagia, hemarthrosis, and bleeding after trauma or surgery.