Paroxysmal nocturnal hemoglobinuria

Alphabetical Test listing

Paroxysmal nocturnal hemoglobinuria-2323

Test name:

Test number:

2323

Excellian order number:

LAB2323

Abbreviation:

PNH

Alternate names:

Acid Hemolysin
CD59
CD157
Ham-Crosby sugar water test
FLAER

Useful for:

Enumeration of granulocytes, monocytes and RBC's lacking PNH-associated markers

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

4.0 mL

Minimum volume:

3.0 mL

Collection instructions:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Required forms/information:

CBC/differential results are required; include a copy of your results along with the request
Diagnosis must be included on request form

Transport and stability:

Ambient - 72 hours

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Inappropriate timing of collection
Interfering substances
Leaking container

Performing lab:

AHL - Flow Cytometry: F

Days set up:

Mo - Fr

TAT:

48 hrs

Method:

Flow Cytometry

Clinical information:

Tubes and Markers:

Tubes:
RPNH (10C) and WPNH (10C)

Markers:
CD15, CD45, CD59, CD64, CD157, CD235a and FLAER

CPT codes:

88184
88185 x 6
Pathology Fee

Date created:

11/30/2001

Revised date:

02/13/2024

Review date:

01/09/2024