ADAMTS13 activity with reflex inhibitor profile

Alphabetical Test listing

ADAMTS13 activity with reflex inhibitor profile-994

  
ADAMTS13 activity with reflex inhibitor profile
  
994
  
LAB994
  
MSO
  
Metalloprotease
Protease Activity Inhibitor for TTP
von Willebrand Factor Cleaving Protease
Microangiopathy
Thrombotic Microangiopathy
VWF protease activity and inhibitor
  
Testing begins with the ADAMTS13 activity assay to evaluate the percent activity. If the ADAMTS13 activity is less than 30%, the inhibitor titer and ADAMTS13 profile interpretation will be performed.
  

Assisting with the diagnosis of immune or acquired thrombotic thrombocytopenic purpura

  

Fasting preferred

Specimen must be collected prior to replacement therapy

  
Sodium citrate (Na cit) plasma
  

To obtain adequate sample volume please collect 2 tubes:

Lt blue Sodium citrate (Na Cit) - 2.7mL 

  
2 mL plasma in 2 plastic vials each containing 1 mL
  
  • Do not over or under fill tube as the ratio of anticoagulant to whole blood is critical

Coag – tube fill guidelines

  • Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting
  
  1. Centrifuge 
  2. Transfer plasma into a plastic vial 
  3. Centrifuge a second time
  4. Aliquot plasma (one mL per aliquot) into two seperate screw cap transfer vial/tube (Mayo T914), leaving 0.25 mL in the bottom of the centrifuged vial
  5. Freeze plasma immediately (no longer than 4 hours after collection) at -20 degrees C or, ideally, below -40 degrees C.
  
  

Frozen (strict) 

Refrigerated - NO

Ambient - NO

  
7 days
  
Mayo Clinic Laboratories (ADAMS): R-NX
  
Mo - Fr, Su
  
3 days
  

Fluorescence Resonance Energy Transfer (FRET)

  

ADAMTS13 ACTIVITY ASSAY: > or =70%

ADAMTS13 INHIBITOR TITER: <0.5 BU

  

Less than 10% ADAMTS13 activity is highly indicative of thrombotic thrombocytopenic purpura (TTP) in an appropriate clinical setting. The presence of ADAMTS13 measurable Bethesda titer is most consistent with an acquired (autoimmune) TTP.

  
85397
85335 (if appropriate)
  
05/31/2022
  
05/24/2024
  
02/28/2023