Plasma Cell Dyscrasia by FISH panel
Plasma Cell Dyscrasia by FISH panel-4280H (blood) or 4280G (bone marrow)
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
Myeloma
Plasma Cell Myeloma
Myeloma FISH panel
14q32.3 rearrangement
TP53 deletion
17p13.1 deletion
1q21.3 gain
1p32.3 loss
MYC rearrangement
- P18
- MYC
- TP53
- IGH with reflex to:
- CCND1/IGH
- IGH/MAF
- IGH/MAFB
- CCND3/IGH
- FGFR3/IGH
Detecting abnormalities of plasma cell dyscrasias, including Plasma Cell Myeloma and to provide myeloma cytogenetic risk stratification information on current Mayo mSMART classification system.
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting
Dk green Sodium heparin (Na hep), no gel
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type
Bone marrow
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
- Only one specimen tube is required to perform all ordered cytogenetics testing.
- The more testing requested, the closer to the optimal volume requirements is required.
EDTA whole blood:
Lavender (EDTA), 4mL
Bone marrow:
Sterile vial/tube
Dk green Sodium heparin (Na hep), no gel
- AHL Cytogenetics oncology request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Leaking container
Fluorescence in situ hybridization (FISH) on CD138 plasma cell enriched specimen
Normal or Abnormal with interpretation
88271 x 8
88275 x 4
Abnormal with a CCND1/IGH reflex
88271 x 10
88275 x 5
Abnormal with a full IGH reflex:
88271 x 18
88275 x 9