Coxiella burnetii (Q Fever), Molecular Detection, PCR, Serum

Alphabetical Test listing

Coxiella burnetii (Q Fever), Molecular Detection, PCR, Serum-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

Q Fever
C. burnetii
Coxiella burnetii

Useful for:

Aiding in the diagnosis of Coxiella burnetii infection (ie, Q fever) using serum specimens

Specimen type:

Serum

Collection container:

Red serum vial/tube - 5 mL

Volume:

1 mL

Minimum volume:

0.5 mL

Collection instructions:

Immediately following collection, mix sample by gently inverting 5 times

Processing instructions:

Allow sample to clot for a minimum of 30 minutes
Centrifuge within two (2) hours of collection
Transfer the serum to a Screw cap transfer vial/tube (Mayo T914), labelled as serum
Freeze

Transport container:

Screw cap transfer vial/tube (Mayo T914)

Alternate collection container:

Gold serum separator (SST) tube

Alternate collection instructions:

Mix sample by gentle inversion, 5 times

Alternate processing instructions:

Allow sample to clot for a minimum of 30 minutes
Spin within two (2) hours of sample collection

Transport and stability:

Frozen (preferred) - 7 days

Refrigerated - 7 days

Specimen retention time:

1 week

Performing lab:

Mayo Clinic Laboratories (CBSRP): R-NX

Days set up:

Mo, We, Fr

TAT:

2 - 7 days

Method:

Real-Time Polymerase Chain Reaction (PCR)

Reference ranges:

Negative

Clinical information:

A positive result indicates the presence of Coxiella burnetii DNA.

A negative result indicates the absence of detectable C burnetii DNA, but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of C burnetii DNA in quantities less than the limit of detection of the assay.

CPT codes:

87798

LOINC code:

90443-3; 31208-2

Date created:

02/08/2023

Review date:

02/08/2023