S-100 by IHC

Alphabetical Test listing

S-100 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

S-100 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Tests included:

All IHC stains will include a positive control tissue

Useful for:

Using polyclonal antibodies, a variety of human tissue types are recognized:
- αα– myocardial and skeletal muscle cells
- αβ– glial cells, melanocytes, chondrocytes, and adnexal glands
- ββ– Schwann cells and Langerhans cells
Pattern of S100 reactivity:

Normal cells	Tumors
Melanocytes	Melanomas and various nevi
Astrocytes	Gliomas
Neurons, neuroblasts	Neuroblastomas
Chondrocytes	Chondrosarcoma and chondromas
Schwann cells	Schwannoma, granular cells tumor, neurofibroma
Fat cells	Liposarcoma
Myoepithelial and duct cells	Subsets of salv gland, sweat gland, and breast tumors
Langerhans cells	Histiocytosis X
Histiocytes and reticular cells	Malignant histiocytosis
Notochord remnant cells	Chordomas

S-100 protein is the most sensitive marker for melanoma; it stains amelanotic melanomas more strongly than pigmented tumors and usually detects desmoplastic and spindled melanomas (which are usually HMB45 negative)
For melanomas, order both S-100 and HMB45 (if lesion is heavily pigmented, consider specifying the alkaline phosphatase (red) staining method.

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

S-100 is a calcium binding protein that is composed of two subunits (alpha or beta) found in 3 different dimer forms.

Staining pattern

Cytoplasmic and nuclear based staining (should see both)

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

08/08/2017

Revised date:

10/17/2018

Review date:

01/12/2024