Granulocyte Antibody Screen, Serum

Alphabetical Test listing

Granulocyte Antibody Screen, Serum-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

Granulocyte Ab
Granulocyte Binding IgG
Leukoagglutinin
Neutrophil Antibodies
LAGGN
LAGGT

Useful for:

Work-up of individuals with autoimmune neutropenia
Work-up of individuals having febrile nonhemolytic transfusion reactions
Work-up for alloimmune neonatal neutropenia

Limitations:

This test is not useful for the diagnosis of neutropenia due to marrow suppression by drugs or tumors.

Specimen type:

Serum

Collection container:

Red serum vial/tube, 5 mL

Volume:

1.5 mL

Minimum volume:

0.3 mL

Collection instructions:

Immediately following collection, mix sample by gently inverting 5 times

Processing instructions:

Allow sample to clot for a minimum of 30 minutes
Spin within two (2) hours of sample collection
Transfer the serum to a Screw cap transfer vial/tube (Mayo T914), labelled as serum

Transport container:

Screw cap transfer vial/tube (Mayo T914)

Transport and stability:

Refrigerated (preferred) - 30 days

Ambient - 7 days

Frozen - 1 year

Reason for rejection:

Specimen collected after a transfusion reaction
SST or serum gel tubes
Gross hemolysis

Performing lab:

Mayo Clinic Laboratories (LAGGN); R-NX

Days set up:

Tu, Th

TAT:

7 - 15 days

Method:

Flow Cytometry/Agglutination

Reference ranges:

Negative

Clinical information:

Granulocyte antibodies are induced by pregnancy, prior transfusion, or transplants. These antibodies can cause neutropenia in various autoimmune disorders. Febrile nonhemolytic transfusion reactions and alloimmune neonatal neutropenia may also be caused by granulocyte associated antibodies, including anti-human leukocyte antigen antibodies.

CPT codes:

86021 x2

Date created:

12/01/2025

Review date:

12/01/2025