FOXO1 by FISH

Alphabetical Test listing

FOXO1 by FISH-4280J

Test name:

FOXO1 by FISH

Test number:

4280J

Excellian order number:

LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES

Abbreviation:

CG FOXO1

Alternate names:

Alveolar rhabdomyosarcoma
FOXO1
13q14

Useful for:

Chromosomal rearrangements involving the FOXO1 gene (located at G-band region 13q14) are recurrent findings in cases of alveolar rhabdomyosarcoma. Two translocations, t(2;13)(q35;q14) and t(1;13)(p36;q14), juxtapose portions of the PAX3 or PAX7 gene (on chromosomes 2 or 1, respectively) with portions of the FOXO1 gene.

Specimen type:

Tumor tissue (solid tumor or other)

Collection container:

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

Volume:

5 mm (3)

Collection instructions:

Specimens should be immersed in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of procedure

Alternate specimen type:

Tumor tissue FFPE block
Tumor tissue FFPE or slides

Alternate specimen volume:

Block
Slides: 2 plus one H & E stained slide

Alternate processing instructions:

Fix the tissue in 10% formalin for 6 - 72 hours.
Prepare a Formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

If the entire block is submitted, an H&E will be cut and stained, and assessed by an HPA pathologist.

Alternate transport container:

FFPE tissue block or

Tissue section mounted on charged, unstained slides

Required forms/information:

Cytogenetics oncology requisition.

Transport and stability:

Ambient

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

As needed

TAT:

5 days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Positive for rearrangement: >1.5% of 200 nuclei

Negative for rearrangement: ≤1.5% of 200 nuclei

CPT codes:

88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Date created:

03/11/2021

Review date:

04/06/2021