CD21 by IHC

Alphabetical Test listing

CD21 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

CD21 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Tests included:

All IHC stains will include a positive control tissue

Useful for:

CD21 is often used to identify the follicular dendritic cell network in follicular lymphomas, and in follicular dendritic sarcomas
In T-cell lymphomas, this antibody is used to identify the hyperplastic dendritic islands in angioimmunoblastic T-cell lymphomas, and in some cases of pleomorphic T-cell lymphomas
CD21 will also illustrate the follicular dendritic meshwork in reactive germinal centers seen in normal and hyperplastic lymph nodes, as well as reactive germinal centers in some lymphomas (i.e. mantel cell lymphoma, MALT lymphoma)
Precursor B and T cell lymphomas (lymphoblastic lymphomas), Burkitt’s lymphomas, plasmacytomas and hairy cell leukemias lack follicular dendritic cells and will thus be CD21 negative
CD21 can also identify the follicular dendritic cell meshwork in nodular lymphocyte-predominate Hodgkin’s disease

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded tissue

Transport container:

Formalin-fixed, paraffin-embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

CD21 is a trans-membrane 145 kDa glycoprotein in the family of complement regulatory proteins, expressed on some mature B cells and on follicular dendritic cells
CD21 is a receptor for complement C3d, which mediates the phagocytosis of complement-coated particles
CD21 is also a receptor for EBV

Staining pattern

Cell surface membrane staining

References

Chan JKC: Proliferative lesions of follicular dendritic cells: An overview, including a detailed account of follicular dendritic cell sarcoma, a neoplasm with many faces and uncommon etiologic associations. Adv Anat Pathol, 4, 6; 387-411, 1997.
Mason DY et al: Immunohistochemical analysis of monoclonal anti B-cell antibodies. Leukocyte Typing II, Vol. 2, Human B-lymphocytes. Springer-Verlag, 1986; 245-55.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

06/16/2017

Revised date:

10/17/2018

Review date:

01/12/2024