DAT Negative Evaluation (Super DAT)

Alphabetical Test listing

DAT Negative Evaluation (Super DAT)-6994

  
DAT Negative Evaluation (Super DAT)
  
6994
  
LAB6994
  
Blood Bank miscellaneous
DAT negative hemolytic anemia evaluation
Super coombs
  
  • Traditional Tube Method:
    • Polyspecific DAT -IgG + C3
    • Monospecific DAT - IgG
    • Monospecific DAT - C3
  • LISS wash solution using traditional tube method:
    • Polyspecific DAT - IgG + C3
    • Monospecific DAT - IgG
    • Monospecific DAT - C3
  • Gel column agglutination: Monospecific DAT - IgG
  • Solid phase: Monospecific DAT - IgG
    •   

    To help with the investigation of Immune-Mediated Red Cell Destruction when the DAT is negative.

      

    Specimens should be tested within 48 hours of collection for optimal results.

    Older specimens (beyond 48 hours) may show decreased sensitivity due to cell deterioration and potential antibody elution

    Complement detection is optimal within 24 hours of collection; older specimens may not accurately reflect in vivo complement binding.

    The Direct Antiglobulin Test detects IgG and/or complement components bound to red blood cells but does not determine the cause of sensitization. Further clinical correlation and additional testing may be required.

    False-negative results may occur due to improper specimen handling, insufficient sensitization, or low-affinity antibodies that dissociate during washing.

    Gel and solid-phase methods may be more sensitive but could detect low levels of bound immunoglobulins with unclear clinical significance.

    The LISS wash method may enhance weakly bound antibodies

      
    EDTA whole blood
      

    Lavender (EDTA), 4mL x 2

    OR

    Lavender (EDTA), 10mL

      
    10.0 mL
      
    5.0 mL
      

    Use aseptic technique

    Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

    Collect specimen Su - Th

    Labelling instructions:

    1. Patient first and last name (Use legal name.)
    2. Patient date of birth
    3. Medical record number optional
    4. Date of sample collection
    5. Time of collection 
    6. Phlebotomist identifier
      

    Do not spin.  Send entire specimen.

      

    Lavender (EDTA), 4mL x 2

    OR

    Lavender (EDTA), 10mL

      

    Ambient (preferred) - 48 hours|(also acceptable <7 days)

    Refrigerated - NO

    Frozen - NO

      
    14 days
      
    • Positie DAT prior to sending to reference lab
    • Improper label (unlabeled or mislabeled)
    • Insufficeint sample volume
      
    Community Blood Center of Greater Kansas City via Memorial Blood Center
      
    Mo - Fr
      
    3 -5 days
      

    Serological

      

    Negative

      

    It is not recommended to collect a sample for Super DAT on a Friday due to transport requirement. A Friday collection would mean the tube received for testing would not be tested within the optimal time to test.

    Recommend sample collection Sunday through Thursday.

     

      
    86880 x 8, 86999
      

    1007-4

      
    09/13/2005
      
    03/17/2025
      
    01/03/2024