7q31 Deletion/Monosomy 7 by FISH

Alphabetical Test listing

7q31 Deletion/Monosomy 7 by FISH-4280G (bone marrow) or 4280H (blood)

Test name:

Test number:

4280G (bone marrow) or 4280H (blood)

Excellian order number:

LAB4280G - CYTOGENETIC BONE MARROW STUDIES
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES

Abbreviation:

7Q-

Alternate names:

AML
Acute Myeloid Leukemia
Deletion 7q31
MDS
Monosomy 7
Myelodysplastic syndrome
7q-
FISH
Fluorescence In Situ Hybridization
MPN
Myeloproliferative Neoplasm

Useful for:

Loss/deletions of 7q are associated with myelodysplastic syndromes (MDS), chronic myeloproliferative neoplasms, particularly idiopathic myelofibrosis (IMF) and acute myeloid leukemia (AML). They are also frequent abnormalities in therapy-related MDS and AML, following previous exposure to alkylating agents. According to current NCCN AML guidelines, a -7 result is classified as a poor/adverse cytogenetic risk factor. In MDS, a -7/ 7q- result is classified as a poor cytogenetic risk factor. In MPN -7/7q- is classified as an unfavorable cytogenetic risk factor.

This test can be ordered individually or as part of the AML or MDS FISH Panels. See these panel tests for more details

Specimen type:

Bone Marrow aspirate

Collection container:

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep ), no gel
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Volume:

5 mL

Minimum volume:

3 mL

Collection instructions:

Transfer aspirate from syringe to transport container.

Processing instructions:

Submit entire specimen unspun.

Transport container:

Sterile vial/tube

Dk green Sodium heparin (Na hep ), no gel

Alternate specimen type:

Bone Marrow Core
Whole blood
Touch Prep Slides

Alternate collection container:

Bone Marrow Core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep ), no gel

Alternate specimen volume:

Bone Marrow core: 10 mm
Whole blood: 5 mL

Alternate collection instructions:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

Alternate processing instructions:

Submit entire specimen unspun.

Alternate transport container:

Sterile tube/cup

Dk green Sodium heparin (Na hep l ), no gel

Required forms/information:

AHL Cytogenetics Oncology Request

Transport and stability:

Ambient

Specimen retention time:

Specimens retained until completion of all testing

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Delay in transport
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

Mo - Fr

TAT:

7 business days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Normal or Abnormal with interpretation.

CPT codes:

88271 x 2
88275
88291

Subject to medical necessity (Y/N):

Yes

LCD/NCD:

Medical necessity

Date created:

03/23/2021

Revised date:

10/03/2022