Body fluid

• Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
• Do not add anticoagulant or holding media
• The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
• Include representative slides (will be returned)

Bone marrow

• Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
• Specimens collected in EDTA are acceptable, but not preferred
• Transfer to an anticoagulant free sterile tube
• If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
• Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

• Collect 5 mL (minimum 1 mL) in a sterile vial/container.
• Do NOT add any anticoagulant or holding media
• The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
• Send representative slides (will be returned)

Tissue

• Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
• Send representative slides (will be returned)