ROS1 by FISH (Lung cancer)

Alphabetical Test listing

ROS1 by FISH (Lung cancer)-4280J

Test name:

Test number:

4280J

Excellian order number:

LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES

Abbreviation:

CG ROS1

Alternate names:

Non-Small Cell Lung Cancer (NSCLC)
6q22

Useful for:

To Identify patients with late-stage, non-small cell lung cancers who may benefit from treatment with Xalkori (Crizotinib).

The ROS1 gene, a close evolutionary relative of ALK, encodes a receptor tyrosine kinase that activates some of the same signaling pathways as ALK. ROS1 rearrangements lead to constitutively active fusion proteins and are detected in 1% to 2% of NSCLC cases.

Specimen type:

Malignant tumor tissue

Collection instructions:

Collect 5 mm³sample of involved tissue (solid tumor or other) and place in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within 1 hr of procedure

Alternate collection container:

Submit 2 unstained 5 micron cuts of involved tissue mounted on positively charged slides and 1 H & E

a formalin-fixed, paraffin-embedded (FFPE) tumor block. An H & E will be cut and stained, and assessed by an HPA pathologist.

Specimen should be fixed in 10% neutral buffered formalin for 6 - 72 hours

Alternate transport container:

FFPE block or slides

Required forms/information:

Cytogenetics oncology requisition or pathology Add-on form
Document time in fixation – specimen should be fixed in 10% neutral buffered formalin for 6 - 72 hours

Transport and stability:

Ambient

Specimen retention time:

Slides - 6 months for slides
Blocks will be returned post reporting

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

As needede

TAT:

5 days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

>15% of nuclei scored displaying rearrangement a minimum of one 5’ and one 3’ ROS1, or a deletion of one 5’ROS1 only.

Results will be reported as either Positive or Negative for ROS1 rearrangement, or Uninformative.

CPT codes:

88377 – Quantitative Morphometric FISH.

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Date created:

03/09/2021

Review date:

04/06/2021