ETV6/RUNX1 by FISH

Alphabetical Test listing

ETV6/RUNX1 by FISH-4280G (bone marrow) or 4280H (blood)

  
ETV6/RUNX1 by FISH
  
4280G (bone marrow) or 4280H (blood)
  
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
  
ETV6/RUNX1
  
Acute Lymphoblastic Leukemia/lymphoma
B-ALL
B-cell ALL
iAMP21
FISH
Fluorescence in situ hybridization
TEL/AML
t(12;21)
  

ETV6/RUNX1 or TEL/AML or t(12;21), or amplification of RUNX1 (iAMP21) rearrangements are recurring abnormalities in B-cell ALL. According to current NCCN B-ALL guidelines, ETV6/RUNX1 or TEL/AML or t(12;21) abnormalities are classified as a good cytogenetic risk factor, and amplification of RUNX1 (iAMP21) is classified as a poor cytogenetic risk factor.

This test can be ordered individually or as part of the B-ALL FISH Panel. See the panel test for more details.

  
Bone marrow aspirate
  

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

  1. Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
  2. Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

  
5 mL
  
3 mL
  

Transfer aspirate from syringe to transport container. 

  

Submit entire specimen unspun.

  

Sterile vial/tube

  

or

Dk green Sodium heparin (Na hep), no gel

  
Bone Marrow core
Sodium heparinized whole blood
Touch Prep slides
  

Bone Marrow Core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

or 

Whole blood:

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow core: 10 mm
Whole blood: 5 mL
  

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

  

Submit entire specimen unspun

  
  

AHL Cytogenetics Oncology Request

  

Ambient

  
Specimens retained until completion of all testing
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Delay in transport
  • Improper storage
  • Leaking container
  • Slides broken beyond repair
  
AHL - Cytogenetics: X
  
Mo - Fr
  
5 business days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

 

  
88271 x 2
88275
88291
  
Yes
  
  
03/23/2021
  
09/13/2022