Hepatitis B Virus (HBV) Genotyping Plus Drug Resistance

Alphabetical Test listing

Hepatitis B Virus (HBV) Genotyping Plus Drug Resistance-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

HBV Genotype
PreCore and Resistance HBV Resistance Testing

Useful for:

Data generated in the genotyping assay is subsequently analyzed for mutations in the reverse transcriptase gene of HBV associated with resistance to therapeutic agents, and if identified an interpretation of their clinical significance provided.

Limitations:

This assay may not be successful when the HBV viral load is <500 IU/mL

Specimen type:

Plasma

Collection container:

Lavender (EDTA), 4mL

Volume:

1.2 mL

Minimum volume:

0.6 mL

Collection instructions:

Immediatley following collection, mix sample by inverting 5 times

Processing instructions:

Centrifuge sample within six hours of collection
Transfer plasma or serum to a Labcorp transport vial and freeze

Transport container:

Labcorp freeze vial (purple cap)

Alternate specimen type:

Serum or plasma

Alternate collection container:

Gold serum separator (SST) tube

Yellow ACD (A or B)

Alternate collection instructions:

Immediatley following collection, mix sample by inverting 5 times

Alternate processing instructions:

Allow SST to clot for 30 minutes before spinning
Centrifuge sample within six hours of collection
Transfer plasma or serum to a Labcorp transport vial and freeze

Transport and stability:

Frozen (preferred) – 14 days

Refrigerated- not accepted

Ambient - not accepted

Reason for rejection:

Quantity not sufficient for analysis
Heparin/SPS plasma (wrong anticoagulant)
Gross hemolysis
Specimen not frozen

Performing lab:

LabCorp (551750) R-LC

Days set up:

2 times per week

TAT:

12 days

Method:

Polymerase chain reaction (PCR) amplification and DNA sequencing

Reference ranges:

An interpretive report will be provided

CPT codes:

87900
87912

Date created:

08/26/2022

Review date:

08/26/2022