KIT Asp816Val mutation analysis
KIT Asp816Val mutation analysis-994
D816V
Systemic mastocytosis mutation
tumors of hematopoietic tissue
Diagnosing systemic mastocytosis using blood or bone marrow specimens
Immediatley following collection, mix sample by inverting 8 – 10 times to prevent clotting
Do not spin
Extracted DNA from blood or bone marrow
Yellow ACD (A or B)
Extracted DNA - 50 µL at 20 ng/µL concentration
Bone marrow:
Lavender (EDTA), 4mL
Yellow ACD (A or B)
Extracted DNA:
1.5 to 2mL screw-top tube (extracted DNA)
Provide indication of volume and concentration of DNA extracted
Molecular Medicare billing request
Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medicare billing request form to notify us of the need for Allina Health Laboratory to bill insurance.
Peripheral blood or bone marrow:
Ambient (preferred) – 14 days
Refrigerated – 14 days
Frozen – NO
Extracted DNA from blood or bone marrow:
Frozen (preferred)
Refrigerated (OK)
Ambient (OK)
DNA: 3 months
- Gross hemolysis
- Moderately to severely clotted
- Bone marrow biopsies
- Paraffin-embedded bone marrow clots
- Paraffin-embedded tissue
- Slides
- Paraffin shavings
Droplet Digital Polymerase Chain Reaction (ddPCR)
An interpretive report will be provided indicating the mutation status as positive or negative.
Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medicare billing request form to notify us of the need for Allina Health Laboratory to bill insurance.