Cytokeratin 5/6 by IHC

Alphabetical Test listing

Cytokeratin 5/6 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

CK 5/6

Tests included:

All IHC stains will include a positive control tissue

Useful for:

This antibody is useful in identifying hyperplastic breast epithelium (CK5/6 positive)
Neoplastic breast proliferations (ADH, DCIS) are typically CK5/6 negative
CK5/6 also is a marker of breast myoepithelial cells
This antibody will also stain squamous epithelium of normal skin
CK5/6 is useful in separating mesotheliomas from pulmonary adenocarcinomas; it will stain mesothelial cells and mesotheliomas, but it is typically negative in adenocarcinomas

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded tissue

Transport container:

Formalin-fixed, paraffin-embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

CK5/6 is an antibody cocktail that reacts with high-molecular weight keratins (56,58 kD)

Staining pattern

Cytoplasmic

References

Cury PM et al; Mod Pathol 2000;13:107-12.
Otterback F et al: Histopathology 2000;37:232-40.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

10/31/2017

Revised date:

10/18/2018

Review date:

01/12/2024