PDGFRB by FISH
PDGFRB by FISH-4280G (bone marrow) or 4280H (blood)
LAB4280H - CYTOGENETIC BLOOD STUDIES
Eosinophilia FISH panel
PDGFRB
PDGFRB rearrangement
t(5;12)
5q31-5q33
FISH
Fluorescence In Situ Hybridization
Identifying patients with chronic myelomonocytic leukemia and other hematologic disorders who may be responsive to imatinib mesylate.
The Platelet-derived growth factor receptor-beta (PDGFRB) produces a tyrosine kinase involved in cell proliferation. PDGFRB has over 30 known partner genes, however the 5;12 translocation involving ETV6 is the most common. Patients with this translocation often have associated hypereosinophilia.
This test can be ordered individually or as part of an Eosinophilia FISH Panel (see the individual panel test for more details).
Bone marrow aspirate:
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
Transfer aspirate from syringe to transport container.
Submit entire specimen unspun.
Sodium heparin (Na heparin) whole blood
Touch prep slides
Bone marrow core:
Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline
Sodium heparin (Na heparin) whole blood:
Dk green Sodium heparin (Na hep), no gel
Sodium heparin (Na heparin) whole blood: 5 mL
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting.
Bone marrow core:
Sterile tube/cup
Whole blood:
Dk green Sodium heparin (Na hep), no gel
AHL Cytogenetics Oncology Requisition
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Delay in transport
- Improper storage
- Leaking container
- Slides broken beyond repair
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation