B-ALL panel by FISH
B-ALL panel by FISH-Order individual components: 4280H (blood) or 4280G (bone marrow)
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
MLL
4/10
ETV/RUNX1
B-cell ALL
iAMP21he
FISH
Fluorescence in situ hybridization
Mixed Lineage Leukemia
Trisomy 4
Trisomy 10
TEL/AML
t(12;21)
t(11q23;?)
The B-ALL FISH Panel is useful for detecting the prognostically significant cytogenetics FISH abnormalities used in the both the pediatric and adult B-Cell ALL according to the NCCN guidelines.
Immediately after collection, mix tube thoroughly by gentle inversion, 8 - 10 times, to prevent clotting
Dk green Sodium heparin (Na hep), no gel
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
Bone marrow
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
EDTA whole blood:
Lavender (EDTA), 4mL
Bone marrow:
Sterile vial/tube
Dk green Sodium heparin (Na hep), no gel
- AHL Cytogenetics oncology request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Leaking container
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation.
Aneuploidy of chromosomes 4 and 10 and t(12;21) ETV6/RUNX1 rearrangements are classified as favorable cytogenetics prognostic indicators in B-ALL.
BCR/ABL1 and MLL rearrangements and intrachromosomal amplification of chromosome 21 (iAMP21) are unfavorable cytogenetics prognostic indicators in B-ALL.