Dihydrorhodamine flow cytometric phorbol myristate acetate test, blood

Alphabetical Test listing

Dihydrorhodamine flow cytometric phorbol myristate acetate test, blood-994

Restricted test:

See specimen requirements prior to placing an order or collection of any specimens.

  
Dihydrorhodamine flow cytometric phorbol myristate acetate test, blood
  
994
  
LAB994
  
MSO
  
Chronic granulomatous disease (CGD)
Neutrophil oxidative burst (NOXB)
Nitroblue tetrazolium (NBT) assay
Chemiluminescence
Dihydrorhodamine (DHR)
Neut
  
  • Diagnosis of chronic granulomatous disease (CGD), X-linked and autosomal recessive forms, complete myeloperoxidase (MPO) deficiency; monitoring chimerism and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase function posthematopoietic cell transplantation
  • Assessing residual NADPH oxidase activity pretransplant
  • Identification of carrier females for X-linked CGD; assessment of changes in lyonization with age in carrier females
  
Sodium heparin (Na hep) whole blood
  

Dk green Sodium heparin (Na hep), no gel

  
5.0 mL
  
1.0 mL
  

Allina facility special instructions:

  1. Due to special requirements for collection, this test requires pathologist pre-approval by calling Client Services at (612) 863-4678. 
  2. If approved, collection must be scheduled in advance and is only performed at the Abbott-Northwestern, Mercy and United Hospital sites.
  3. This test is performed by Mayo Clinic Laboratories (MCL). If it is more convenient for them, patients may go to a Mayo facility for specimen collection.

 

  • Both a patient and control specimen from an unrelated, healthy donor are required.
    • Control specimen should be collected from an Allina Health employee.
  • Collect specimens Mo - Th only. No weekends or holidays.

Collection instructions:

  1. Draw a control specimen from a normal (healthy), unrelated person within an hour of the patient.
  2. Immediately following collection, mix sample thoroughly by gently inverting 5 - 10 times, to prevent clotting
  3. Label clearly on outermost label normal control.
  

It is recommended that specimens arrive within 24 hours of specimen collection.

  

Dk green Sodium heparin (Na hep), no gel

  

Ambient (strict) – 48 hours

Refrigerated - NO

Frozen - NO

  
4 days
  
  • Gross hemolysis
  • Gross lipemia
  
Mayo Clinic Laboratories (DHRP): R-NX
  
Mo - Fr
  
4 days
  

Flow cytometry

  

Result name

Unit

Cutoff for defining normal

% PMA ox-DHR+

%

≥95%

MFI PMA ox-DHR+

MFI

≥60

Control % PMA ox-DHR+

%

≥95%

Control MFI PMA ox-DHR+

MFI

≥60

 

The appropriate age-related reference values for Absolute Neutrophil Count will be provided on the report.

  
86352
  
01/27/2020
  
03/15/2023
  
01/02/2024