Functional testing of red blood cell sensitivity to osmotic stress
Do not use to distinguish between spherocytes in hereditary spherocytosis and acquired autoimmune hemolytic anemia
Draw Mon - Wed ONLY
Immediatley following collection, mix sample by inverting 8 - 10 times to prevent clotting.
Lavender (EDTA), 4mL
Immediately following collection, mix sample by inverting 5 - 10 times to prevent clotting.
Dk green heparin (Li or Na), no gel
Refrigerated (strict) - 72 hours
Ambient - NO
Frozen - NO
Specimens must be received and testing performed by ARUP within 72 hours of collection.
Spectrophotometry
See report
For patients with acute hemolysis, a normal red cell osmotic fragility test result cannot exclude an osmotic fragility abnormality since the osmotically labile cells may be hemolyzed and not present. Recommend testing during a state of prolonged homeostasis with stable hematocrit.