Prostate cocktail by IHC

Alphabetical Test listing

Prostate cocktail by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

P504S/AMCR
P63

Tests included:

All IHC stains will include a positive control tissue

Useful for:

Prostate cocktail can be used to discriminate prostatic adenocarcinoma from high-grade PIN, and from benign glands
Important exceptions: in our lab, P504S does stain seminal vesicles and ejaculatory ducts. Therefore, it is important to evaluate for pigment (which would be consistent with either ejaculatory ducts or seminal vesicles) before interpreting the P504S
P504S is not specific for prostate, and therefore should only be used on prostate tissues. Other tissues that can stain for P504S include

Prostate cancer vs HGPIN

	p504S (racemase)	p63
Cancer	+	-
HGPIN	+	+
Benign	-	-

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Antibody

Cocktail composed of antibodies to P504S/ Alpha-methlylacyl-CoA Racemase (AMACR) and P63

Specifications

Prostate cocktail contains both P504S and P63 to simultaneously evaluate for potentially malignant prostate glands and the outer basal cell layer of glands
P504S is a prostate cancer specific gene found in prostatic adenocarcinomas (82-100% of cases) and high-grade PIN (64%)
P504S is usually not present (or is focal and weak) in benign prostatic tissues
P63 is a marker of myoepithelial cells (which should be present in benign glands and in high grade PIN)

Staining pattern

Dual stain, with red and brown chromogens showing:

Red: Strong granular cytoplasmic and apical staining for P504S, with circumferential staining of malignant glands
Brown: nuclear staining for P63 in myoepithelial cells

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

10/31/2017

Revised date:

10/19/2018

Review date:

01/12/2024