Fungus/Pneumocystis stain

Alphabetical Test listing

Fungus/Pneumocystis stain-6301

Test name:

Test number:

6301

Alternate names:

APN
Organism eval for: Aspergillus, Candida, GMS, PCP, P. carinii, jirovecii, yeast
PAS stain
Periodic acid-Schiff stain
Pneumocystis DFA
Silver stain

Limitations:

The following specimen types should not be submitted for Fungal/Pneumocystis stain:

Skin, Hair, Nails:

See KOH prep – skin, hair, nails (4348) or Fungus culture, skin, hair, nails (888)

Stool

See KOH prep, other sources (6524) or Fungus culture, other sources (6579)

Tissue:

Submit in formalin and order a Path tissue exam. PAS stain can be performed in Histology on skin and nails.

Vaginal, Oral/Mouth:

See KOH Prep, Other Sources (6524) or Fungus Culture, Other Sources (6579)

Collection container:

Sterile container

Collection instructions:

Body Fluid:

Place specimen into a labeled sterile container

Bronchial/Esophageal brushing:

If cultures are ordered: place brush in labeled capped tube containing sterile saline
If no cultures are ordered, place brush into a labeled tube of CytoLyt ™

Bronchial washing/BAL (Bronchoalveolar lavage):

Collect specimen into a labeled sterile mucus trap

Purulent material:

Place specimen into a labeled sterile container or use culture swab
Indicate source

Sputum:

Collect the first early morning deep cough specimen since bronchial secretions have pooled overnight
The patient should be instructed to rinse out the mouth with water only and then raise material from lungs by coughing as deeply as possible
Cough into sterile labeled container
o If no cultures are ordered, have patient cough directly into a container of labeled Cytolyt™

Processing instructions:

Submit entire specimen

Transport container:

Submit per specimen type and collection instructions

Cytolyte

Required forms/information:

Source

Transport and stability:

Refrigerated

Reason for rejection:

Unlabeled or mislabeled specimen
Unlabeled slide in a labeled slide container
Leaking specimen if label has been rendered unreadable
Slide broken beyond repair

Performing lab:

AHL - Cytology: A

Days set up:

Daily

TAT:

1 day

Method:

Microscopic examination

Reference ranges:

No organism or Hyphae identified

CPT codes:

Smear: 88160 and 88312
Fluid: 88122 and 88312

Date created:

04/28/2004

Revised date:

06/21/2019

Review date:

12/15/2023