Hepatitis C virus NS5A drug resistance assay

Alphabetical Test listing

Hepatitis C virus NS5A drug resistance assay-994

  
Hepatitis C virus NS5A drug resistance assay
  
994
  
LAB994
  
MSO
  
HCV drug resistance
HCV NS5A drug resistance assay
NS5A resistance analysis
  

Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Assay should be used for patients with documented HCV genotype 1.

  
  • This test may not be successful when the HCV viral load is <500 IU/mL
  • This assay requires an HCV genotype of either 1a or 1b
  
Serum
  

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

  
2.0 mL
  
1.0 mL
  

Immediately following collection, mix sample by gently inverting 5 times

  
  1. Allow sample to clot for a minimum of 30 minutes
  2. Spin within six (6) hours of collection
  3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
  4. Freeze
  

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

  
EDTA plasma
  

Lavender (EDTA) vial/tube

  
  1. Spin 
  2. Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as plasma, within six (6) hours of collection
  3. Freeze
  
  • Patient's HCV genotype (1a or 1b) 
  

Frozen (strict)

  
  • Insufficient volume
  • Heparinized plasma
  •  Non-frozen specimens
  • Specimens exposed to repeated freeze/thaw cycles
  • Specimen received in "pop-top" or "snap- cap" tube
  
Monogram Biosciences via LabCorp (550325): R-NX
  
Varies
  
14 days
  

Polymerase chain reaction (PCR) amplification and DNA next generation sequencing

  

An interpretive report will be provided

  
87902
87900
  
Result 72862-6
  
05/29/2019
  
04/14/2022
  
08/16/2022