HBc total antibody measures both IgG & IgM but when reactive, does not distinguish between the two. If acute infection is suspected, order an Anti-HBc IgM
Immediately following collection, thoroughly mix sample by gently inverting 5 times
Gold serum separator (SST) tube
Lt green/Lavender:
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting
Red:
Lt green PST:
Spin within two (2) hours of sample collection
Lavender:
Plasma separator (Lt green PST)
Refrigerated (preferred) - 14 days
Ambient - 7 days
Frozen - 3 months
Electrochemiluminescence immunoassay (ECLIA)
Non-reactive