Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• NUT gene (NUTM1, NUT midline carcinoma family member 1) on chromosome 15 encodes NUT protein (nuclear protein in testis, FAM22H and NUT family member 1). Function of NUT protein is uncertain.
• NUT carcinoma is a highly aggressive malignancy defined by translocations involving the NUT gene. Fusion with partner genes (BRD4, BRD3, NSD3, and others) leads to the deregulated gene transcription of multiple genes including cMYC and TP63.

 
Staining pattern

Nuclear staining. Cytoplasmic staining is nonspecific.

• NUT carcinoma: Strong speckled nuclear staining in greater than 50% of tumor cells. Most NUT carcinomas demonstrate strong speckled staining in >90% of tumor cells.
• Majority of malignant ovarian germ cell tumors and a subset of other germ cell tumors (seminomas, embryonal carcinoma, and others) demonstrate nuclear staining in a subset of tumor cells. The nuclear staining is uniform (non-speckled) and has been reported to be weak to moderate in intensity.
• Staining in normal tissues: Germ cells of the testis and ovary demonstrate homogenous (non-speckled) nuclear staining.