CBFB by FISH

Alphabetical Test listing

CBFB by FISH-4280G (bone marrow) or 4280H (blood)

Test name:

CBFB by FISH

Test number:

4280G (bone marrow) or 4280H (blood)

Excellian order number:

LAB4280G - CYTOGENETIC BONE MARROW STUDIES
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES

Abbreviation:

CBFB

Alternate names:

Acute Myeloid Leukemia
AML
CBFB-MYH11
FISH
Fluorescence in situ hybridization
inv(16)
t(16;16)

Useful for:

CBFB or inv(16) or t(16;16) are recurring abnormalities in AML. According to current NCCN AML guidelines, these abnormalities are classified as a favorable cytogenetic risk factor.

This test can be ordered individually or as part of the AML FISH Panel. See the panel test for more details.

Specimen type:

Bone marrow aspirate

Collection container:

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep ), no gel
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Volume:

5 mL

Minimum volume:

3 mL

Collection instructions:

Transfer aspirate from syringe to transport container.

Processing instructions:

Submit entire specimen unspun.

Transport container:

Sterile vial/tube

Dk green Sodium heparin (Na hep ), no gel

Alternate specimen type:

Bone Marrow core
Sodium heparinized whole blood
Touch Prep slides

Alternate collection container:

Bone Marrow Core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep ), no gel

Alternate specimen volume:

Bone marrow core: 10 mm
Whole blood: 5 mL

Alternate collection instructions:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

Alternate processing instructions:

Submit entire specimen unspun.

Alternate transport container:

Sterile tube/cup

Dk green Sodium heparin (Na hep l ), no gel

Required forms/information:

AHL Cytogenetics Oncology Request

Transport and stability:

Ambient

Specimen retention time:

Specimens retained until completion of all testing

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Delay in transport
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

Mo - Fr

TAT:

5 business days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Normal or Abnormal with interpretation

CPT codes:

88271 x 2
88275
88291

Subject to medical necessity (Y/N):

Yes

LCD/NCD:

Medical necessity

Date created:

03/23/2021

Revised date:

09/14/2022