Hemoglobinopathy fractionation cascade

Alphabetical Test listing

Hemoglobinopathy fractionation cascade-13520

Test name:

Test number:

13520

Excellian order number:

LAB13520

Abbreviation:

HGFRAC

Alternate names:

Hemoglobin electrophoresis

Tests included:

Hgb F
Hgb A
Hgb A2
Hgb S

Reflex for Hgb Solubility includes:

Hgb C
Hgb E
Hgb Variant

Useful for:

Diagnose thalassemias and hemoglobin variants

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

1.5 mL

Minimum volume:

0.8 mL
Submission of the minimum volume does not allow for repeat testing

Collection instructions:

Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Required forms/information:

State the patient's age, ethnic background, MCV, transfusion history, and any other known hematological data on the test request form.

Transport and stability:

Refrigerated (preferred) – 14 days

Ambient – 14 days

Frozen – 14 days

Freeze/thaw cycles - stable x3

Reason for rejection:

Clotted specimen
Aged specimen
Improperly stored blood samples

Performing lab:

LabCorp (121690): R-LC

Days set up:

Mo - Sa

TAT:

2 - 5 days

Method:

Capillary electrophoresis (CE)

High-pressure liquid chromatography (HPLC)

Hgb Solubility: Sodium hydrosulfite reduction

Reference ranges:

% (see report for interpretation)

CPT codes:

83020

Additional CPT code(s), if appropriate:
85660 - Hgb solubility
or
83021 - Hgb fractionation by HPLC
or
83021 - Hgb fractionation by HPLC
85660 - Hgb solubility

Date created:

03/01/2021

Revised date:

12/21/2023

Review date:

12/21/2023