Complement, total

Alphabetical Test listing

Complement, total-13316

Test name:

Complement, total

Test number:

13316

Excellian order number:

LAB13316

Abbreviation:

CH50

Alternate names:

CH50
Total hemolytic complement

Useful for:

Evaluate and follow up SLE (systemic lupus erythematosus) patient's response to therapy; may predict disease flare in SLE; evaluate for complement component deficiency; evaluate complement activity in cases of immune complex disease, glomerulonephritis, rheumatoid arthritis, SBE, cryoglobulinemia. The CH50 assay mainly evaluates the classical pathway.

Specimen type:

Serum

Collection container:

Gold serum separator (SST) tube

Volume:

1.0 mL

Minimum volume:

0.5 mL

Collection instructions:

Immediately following collection, thoroughly mix sample by gently inverting 5 times

Processing instructions:

Allow sample to clot for a minimum of 30 minutes
Spin within two (2) hours of sample collection
Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
Freeze

Transport container:

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Alternate collection container:

Red serum vial/tube, 5 mL

Alternate processing instructions:

Allow sample to clot
Spin within two (2) hours of sample collection
Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)
Freeze

Transport and stability:

Frozen (strict) - 14 days

Freeze/thaw cycles - stable x 3

Ambient - NO

Refrigerated - NO

Reason for rejection:

Specimen not frozen
Gross lipemia
Plasma specimen

Performing lab:

LabCorp Burlington (001941): R-LC

Days set up:

Su - Fr

TAT:

2 - 4 days

Method:

Quantitative liposome lysis by spectrophotometry

Reference ranges:

Age	Male (U/mL)	Female (U/mL)
1 – 30 d	Not established	Not established
31 d – 6 m	> 32	> 20
7 m – 17 y	> 39	> 39
> 17 y	> 41	> 41

CPT codes:

86162

LOINC code:

Result 4532-8

Date created:

03/29/2019

Revised date:

04/21/2022

Review date:

12/21/2023