Gold serum separator (SST) tube

• For more than one allergen, use this formula to calculate the amount of serum required:

50 µL X (# of allergens) + 250 µL = total amount serum needed

• Immediately following collection, mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Lt green plasma separator (PST)

Red serum vial/tube - 5 mL

Lavender (EDTA), 4mL (acceptable, but not preferred)

Red/grey serum separator (SST) vial/tube, 10 mL

Lt green/lavender:

Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Lt green:

Spin within two (2) hours of sample collection

Red:

1. Allow sample to clot
2. Spin
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL), labelled as serum, within two (2) hours of sample collection

Lavender:

1. Spin
2. Transfer plasma to a False bottom plasma/serum transport vial/tube (AHL), labelled as EDTA plasma, within two (2) hours of sample collection

Lt green PST

False bottom plasma/serum transport vial/tube (AHL)

Red/grey serum separator (SST) vial/tube, 10 mL

Ambient (preferred) - 3 days

Refrigerated - 7 days

Frozen (OK, one time)

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
• Delay in transport
• Improper storage temperature affecting results
• Improper container
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)

Fluorescence Enzyme Immunoassay (FEIA)

<0.35 kU/L

Medical necessity