Coxiella burnetii (Q fever), Molecular Detection, PCR, Tissue

Alphabetical Test listing

Coxiella burnetii (Q fever), Molecular Detection, PCR, Tissue-994

Test name:

Test number:

994

Excellian order number:

LAB994

Useful for:

Aiding in the diagnosis of Coxiella burnetii infection (eg, Q fever) using tissue specimens

Specimen type:

Fresh tissue or biopsy (Lung, bone, liver, heart valve, aorta, or endocardium)

Collection container:

Sterile container

Volume:

Entire collection or 5 mm (3)

Transport container:

Refrigerated (preferred) - 7 days

Frozen - 7 days

Ambient - No

Specimen retention time:

1 week

Reason for rejection:

Tissue in formalin, formaldehyde
Bone marrow
Decalcified bone
Bone marrow slides

Performing lab:

Mayo Clinic Laboratories (CBRP): R-NX

Days set up:

Mo, We, Fr

TAT:

3-7 days

Method:

PCR

Bacterial nucleic acid is extracted from the specimen using the automated MagNA Pure instrument. The purified DNA is placed on the LightCycler instrument, which amplifies and monitors by fluorescence the development of target nucleic sequences after each PCR cycle. A specific target sequence from Coxiella burnetii is amplified and the resulting segment is detected using specific hybridization probes. Detection of the C burnetii target is performed through melting curve analysis using the LightCycler software.

Reference ranges:

Negative

Clinical information:

A positive result indicates the presence of Coxiella burnetii DNA.

A negative result indicates the absence of detectable C burnetii DNA, but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of C burnetii DNA in quantities less than the limit of detection of the assay.

CPT codes:

87797

Date created:

08/13/2022

Revised date:

09/14/2023

Review date:

08/15/2022