TP53 by FISH

Alphabetical Test listing

TP53 by FISH-4280G (bone marrow) or 4280H (blood)

Test name:

TP53 by FISH

Test number:

4280G (bone marrow) or 4280H (blood)

Excellian order number:

LAB4280G - CYTOGENETIC BONE MARROW STUDIES
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES

Abbreviation:

TP53

Alternate names:

Myeloma
Myeloma FISH panel
Plasma Cell Myeloma
TP53 deletion
17p13.1 deletion
FISH
Fluorescence In Situ Hybridization

Useful for:

TP53 deletion is a recurring abnormality in AML, CLL, MDS, and plasma cell dyscrasia disorders including Plasma Cell Myeloma.

TP53 deletion is a poor cytogenetic risk factor across several disorders including AML, CLL, MDS and myeloma.

This test can be ordered individually or as part of a Plasma Cell Dyscrasia FISH Panel (See the individual panel test for more details).

Specimen type:

Bone Marrow aspirate: 5 mL (minimum 3 mL)

Collection container:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

Volume:

5 mL

Minimum volume:

3 mL

Collection instructions:

Transfer aspirate from syringe to transport container.

Processing instructions:

Submit entire specimen unspun.

Transport container:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel

Alternate specimen type:

Bone marrow core
Sodium heparin (Na hep) whole blood
Touch prep slides

Alternate collection container:

Bone marrow core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep), no gel

Alternate specimen volume:

Bone marrow core: 10 mm
Whole blood 5 mL

Alternate collection instructions:

Whole Blood:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting.

Alternate processing instructions:

Submit entire specimen

Alternate transport container:

Bone marrow core:

Sterile vial/tube or cup

Whole Blood:

Dk green Sodium heparin (Na hep), no gel

Required forms/information:

AHL Cytogenetics oncology request

Transport and stability:

Ambient

Specimen retention time:

Specimens retained until completion of all testing

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Delay in transport
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

Mo-Fr

TAT:

14 business days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Normal or Abnormal with interpretation

CPT codes:

88271 x 2
88275
88291

Subject to medical necessity (Y/N):

Yes

LCD/NCD:

Medical necessity

Date created:

03/16/2021

Revised date:

08/29/2022

Review date:

09/08/2022