Atypical Hemolytic Uremic Syndrome complement panel, serum and plasma

Alphabetical Test listing

Atypical Hemolytic Uremic Syndrome complement panel, serum and plasma-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

aHUS
HUS

Useful for:

Detecting deficiencies in the alternative pathway that can cause atypical-hemolytic uremic syndrome, dense deposit disease, and C3 glomerulonephritis
A second-order test that aids in the differential diagnosis of thrombotic microangiopathies

Patient preparation information:

Fasting preferred
- Fasting for a blood test
Samples should not be collected earlier than 48 hours following plasma exchange

Specimen type:

Sodium citrate (Na cit) plasma and serum

Collection container:

Lt blue Sodium citrate (NaCit)

Red serum vial/tube, 5 mL

Volume:

1.5 mL Na Cit plasma and 1.5 mL serum

Minimum volume:

1.0 mL each, Na cit plasma and serum

Collection instructions:

Samples should not be drawn earlier than 48 hours following plasma exchange
Immediatley following collection, mix sample by gentle inversion 3 - 4 times to prevent clotting
Place sample on ice

Processing instructions:

Allow serum sample to clot
Spin cold
Transfer serum and plasma to two (2) Screw cap transfer vial/tubes (Mayo T914)
Freeze within 30 minutes

Transport container:

Two (2) Screw cap transfer vial/tube (Mayo T914) (2), labeled as serum and Na cit plasma

Transport and stability:

Frozen (strict) - 14 days

Reason for rejection:

Gel separator/SST tubes are not acceptable

Performing lab:

Mayo Clinic Laboratories (AHUSD): R-NX

Days set up:

Varies

TAT:

12 - 21 days

Method:

C3HUS, C4HUS, FBCA, FHCA, C5AG2: Nephelometry

COM3: Automated Liposome Lysis Assay

AH503, C4D, CBB, SC5B9: Enzyme-Linked Immunosorbent Assay (ELISA)

Reference ranges:

An interpretive report will be included

Factor B complement Ag:                                     15.2-42.3 mg/dL
SC5b-9 complement:                                                  ≤250 ng/mL
Factor H complement Ag: 18.5-40.8 mg/dL
C4d complement activation fragment:                          ≤9.8 μg/mL
CBb complement activation fragment:                          ≤1.6 μg/mL
Complement C4:                                                        14-40 mg/dL
Complement C3:                                                       75-175 mg/dL
Complement, alternate pathway (AH50), functional:      ≥46% normal
Complement, total:                                                      30-75 U/mL

Reference values have not been established for patients who are <16 years of age.

CPT codes:

86160 x 7
86161
86162

Date created:

09/04/2019

Revised date:

03/09/2023

Review date:

03/08/2023