Bile acids, fractionated and total, LC/MS-MS
Bile acids, fractionated and total, LC/MS-MS-994
This assay uses LC/MS-MS to quantify the free acid, glycine-conjugate and taurine-conjugate forms of cholic acid, chenodeoxycholic acid, deoxycholic acid, and ursodeoxycholic acid.
Patient should be fasting.
Submission of the minimum volume does not allow for repeat testing
Immediately following collection, mix sample by gently inverting 5 times
- Allow sample to clot for a minimum of 30 minutes
- Spin within two (2) hours of sample collection
Gold serum separator (SST) tube
Sodium heparin (Na Hep) plasma
Lavender/Dk Green:
Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting
Red:
- Allow sample to clot
- Spin within two (2) hours of sample collection
- Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum
Lavender/Dk green:
- Spin within two (2) hours of sample collection
- Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as the appropriate plasma type
Refrigerated (preferred) - 3 days
Ambient - 1 days
Frozen - 14 days
- Whole blood
High-pressure liquid chromatography/tandem mass spectrometry (HPLC/MS-MS)
|
Ursodeoxycholic acids |
< 1.9 |
|
Cholic acids |
< 2.2 |
|
Chenodeoxycholic acids |
< 5.8 |
|
Deoxycholic acids |
< 3.3 |
|
Total Bile acids |
< 9.2 |
Bile acids may be measured to investigate obstructive cholestasis in pregnancy. In addition, bile acids are required for cholesterol absorption, fat-soluble vitamin absorption, and to provide resistance to overgrowth of intestinal bacteria. Bile acid levels may be reduced or elevated due to various genetic defects.
503641 Total Bile Acids 14628-2
503642 Ursodeoxycholic Acids 55159-8
503643 Cholic Acids 30518-5
503644 Chenodeoxycholic Acids 30519-3
503645 Deoxycholic Acids 30520-1