CHOP by FISH

Alphabetical Test listing

CHOP by FISH-4280J

Test name:

CHOP by FISH

Test number:

4280J

Excellian order number:

LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES

Abbreviation:

CG CHOP

Alternate names:

CHOP
DDIT3
Myxoid liposarcomas
12q13

Useful for:

Rearrangement of the DDIT3 (CHOP) gene (CHOP) located at 12q13 is the cytogenetic hallmark of myxoid liposarcomas. A t(12;16)(q13;p11) resulting in the fusion of the DDIT3 and FUS genes is present in more than 90% of myxoid and round cell liposarcomas. A variant t(12;22)(q13;q12) resulting in the fusion of the DDIT3 and EWSR1 genes has also been reported.

Specimen type:

Malignant tissue (solid tumor or other)

Collection container:

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Volume:

5 mm (3)

Collection instructions:

Place 5 mm³tissue specimen in a sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one hour of procedure.

Transport container:

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one hour of procedure.

Alternate specimen type:

Tumor tissue FFPE block
Tumor tissue FFPE slides

Alternate collection container:

Formalin vial

Alternate processing instructions:

Fix the tissue in 10% neutral buffered formalin for 6 - 72 hours
Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block (an H&E will be cut and stained, and assessed by an HPA pathologist), or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

Alternate transport container:

Formalin-fixed paraffin-embedded (FFPE) tissue block or 5 micron cuts mounted on positively charged slides

Required forms/information:

Cytogenetics oncology request

Transport and stability:

Ambient

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Delay in transport
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

2 - 3 times per week

TAT:

5 days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Positive for rearrangement: >3.0% of 200 nuclei

Negative for rearrangement: ≤3.0% of 200 nuclei

CPT codes:

88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Date created:

03/11/2021

Review date:

04/06/2021