PAX8 by IHC

Alphabetical Test listing

PAX8 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

PAX8 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Tests included:

All IHC stains will include a positive control tissue

Useful for:

PAX8 is expressed in ovarian serous (99%), endometrioid (98%), and clear cell carcinomas (78%) (and only very rarely in mucinous ovarian tumors), and cervical carcinomas (91%)
PAX8 is present in 90-98% of clear cell renal cell carcinomas, 90% of papillary renal cell carcinomas, and 81-95 % of oncocytomas
PAX8 is expressed in thyroid neoplasms (91%)
No (or focal weak) expression for PAX8 is seen in lung, breast, and non-GYN carcinomas
Thymic carcinomas and thymomas may show moderate staining with PAX8
PAX8 is useful in a panel differentiating peritoneal mesothelioma from papillary serous
PAX8 can be used to discriminate GYN tumors from breast tumors

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

PAX8 is a member of the PAX family of transcription factors that are active in thyroid development
PAX8 expression is seen in thyroid, non-ciliated mucosal cells of fallopian tubes, ovarian inclusion cysts, and in renal tubular cells and associated tumors
PAX8 positivity can be seen in B-cell lymphomas, likely due to cross reactivity with PAX5

Staining pattern

Nuclear

References

Laury AR et al: A Comprehensive Analysis of PAX8 Expression in Human Epithelial Tumors Am J Surg Pathol 2011;35:816-826).
Morgan EA at al: PAX8 and PAX5 are differentially expressed in B-cell and T-cell lymphomas. Histopathology 2013, 62, 406-413.
Moretti L at al: N-terminal PAX8 polyclonal antibody shows cross-reactivity with N-terminal region of PAX5 and is responsible for reports of PAX8 positivity in malignant lymphomas. Modern Pathology 2012, 25, 231-236.
Ordonez NG: Value of PAX 8 Immunostaining in Tumor Diagnosis: A Review and Update. Adv Anat Pathol 2012;19:140-151).

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

08/07/2017

Revised date:

10/19/2018

Review date:

01/12/2024