Karius Test

Alphabetical Test listing

Karius Test-994

Restricted to Allina Health infectious disease clinicians

Test name:

Karius Test

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Useful for:

Provides a non-invasive way to rapidly detect over 1000 pathogens causing both deep-seated and bloodstream infections from a single blood sample.

Limitations:

This is a time sensitive test sent to a third party lab. Other facilities who wish to order a test should work directly with Karius.

Specimen type:

EDTA plasma

Collection container:

Lavender (EDTA), 4mL

Volume:

2.0 mL

Minimum volume:

0.7 mL

Collection instructions:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

Processing instructions:

Centrifuge sample within 24 hours of draw at 1600 x g for 10 minutes.
Transfer plasma to a Screw cap transfer vial/tube (Mayo T914) within 6 hours of collection
Freeze sample

Transport container:

Screw cap transfer vial/tube (Mayo T914)

Transport and stability:

Frozen (preferred) - 6 months

Ambient - 4 days

Reason for rejection:

Plasma not separated from whole blood within 24 hours
Unfrozen sample received more than 4 days after draw
Specimen with incomplete or improper separations of plasma

Performing lab:

Karius Laboratory (MML1359) via Mayo Clinic Laboratories (ZW300); R-NX

Days set up:

Mo - Sa

TAT:

2 - 9 days

Method:

Next-Generation Sequencing (NGS)

Reference ranges:

Varies per organism: * Reference Interval = the 97.5th percentile MPM concentration detected in PPT plasma from a cohort of 684 asymptomatic donors

MPM (* Molecules Per Microliter = number of DNA fragments present in one microliter of plasma)

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

0152U

Date created:

06/19/2023

Revised date:

06/11/2024

Review date:

06/19/2023