Aldolase
Aldolase-13318
Evaluate muscle wasting process.
High levels are found in progressive Duchenne muscular dystrophy (MD). Elevations occur in carriers of MD, in limb-girdle dystrophy and other dystrophies, in dermatomyositis, polymyositis, and trichinosis, but not in neurogenic atrophies (eg, multiple sclerosis or in myasthenia gravis).
Immediatley following collection, mix sample by gently inverting 5 times
- Allow sample to clot
- Spin within 30 minutes of sample collection
Not removing refrigerated specimens from the clot results in aldolase levels 12% to 46% higher.
Gold serum separator (SST) tube
Sodium citrate (Na cit)plasma
Sodium flouride (Na fl) plasma
Heparin plasma
Red:
- Allow sample to clot
- Spin
- Immediately (30 minutes) transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp) labelled as "serum"
Lavender, Lt blue, Grey or Dk green:
- Spin
- Immediately (30 minutes) transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp) labelled as the appropriate type of plasma.
Not removing refrigerated specimens from the clot/cells results in aldolase levels 12% to 46% higher.
Refrigerated (preferred) - 7 days
Ambient - 1 day
Frozen - 15 days
Freeze/thaw cycles - stable x3
- Hemolysis (red cells contain aldolase)
Kinetic - 340 nm at 37ºC
0 - 30 days: Not established
31 days - 1 year: 5.0−11.7 units/L
>1 year: 3.3−10.3 units/L
High levels are found in progressive Duchenne muscular dystrophy (MD). Elevations occur in carriers of MD, in limb-girdle dystrophy and other dystrophies, in dermatomyositis, polymyositis, and trichinosis, but not in neurogenic atrophies (eg, multiple sclerosis or in myasthenia gravis).