CD1a by IHC

Alphabetical Test listing

CD1a by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

CD1a by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Leu-6
OKT6
SKg
T6

Tests included:

All IHC stains will include a positive control tissue

Useful for:

CD1a can be used in conjunction with S-100 to differentiate between the various malignant and benign histiocytic proliferations:

	CD1a	S-100
Langerhans cells (LC)*	+	+
Interdigitating cells	-	+
Dendritic Reticulum cells	-	+

* This pattern of staining is seen in both benign (e.g. dermatopathic lymphadenopathy) and malignant (histiocytic) LC proliferations. However, LC's in histiocytosis X have been found to be CD4 positive, while benign LC's are CD4 negative.

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded tissue

Transport container:

Formalin-fixed, paraffin-embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

CD1a is a 49kDa cell surface glycoprotein found on human Langerhans cells and thymocytes, but not on peripheral T-cells

Staining pattern

Cell membrane and/or diffuse cytoplasmic

References

Ornvold et al, Virchows Archiv-A, 416(5):403-10, 1990.
Shamoto et al, Advances in Exp. Med & Biology, 378:139-41, 1995.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

05/15/2017

Revised date:

10/17/2018

Review date:

01/12/2024