MDS panel by FISH
MDS panel by FISH-Order individual components; 4280H (blood) or 4280G (bone marrow)
MDS panel by FISH
Order individual components; 4280H (blood) or 4280G (bone marrow)
4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
4280G - CYTOGENETIC BONE MARROW STUDIES
4280G - CYTOGENETIC BONE MARROW STUDIES
Deletion 5q31
Deletion 7q31
Deletion 20q
FISH
Fluorescence In Situ Hybridization
MDS
MLL
Monosomy 5
Monosomy 7
Trisomy 8
Myelodysplastic syndrome
t(11q23;?)
5q-
-5
7q-
-7
+8
Deletion 7q31
Deletion 20q
FISH
Fluorescence In Situ Hybridization
MDS
MLL
Monosomy 5
Monosomy 7
Trisomy 8
Myelodysplastic syndrome
t(11q23;?)
5q-
-5
7q-
-7
+8
- 5Q-
- 7Q-
- FGFR1 (+8)
- 20Q-
- MLL
The MDS FISH panel is useful for detecting the recurrent FISH abnormalities associated with MDS NCCN guidelines
Sodium heparin (Na hep) whole blood
5 mL
2.0 mL
- Only one specimen tube is required to perform all ordered cytogenetics testing
- The more testing ordered, the closer to the optimal volume requirement is preferred
- Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting
Dk green Sodium heparin (Na hep), no gel - 4mL
Bone marrow
EDTA whole blood (chromosome analysis study is not recommended for this specimen type)
EDTA whole blood (chromosome analysis study is not recommended for this specimen type)
Bone marrow: 10 mL (minimum 5 mL)
Bone marrow
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
Bone marrow:
Sterile vial/tube
Dk green Sodium heparin (Na hep), no gel
EDTA whole blood:
Lavender (EDTA), 4mL
- AHL Cytogenetics oncology requisition
Ambient
Pellets - 2 years
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Leaking container
AHL - Cytogenetics: X
Mo, We, Fr
5 - 7 days
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation.
- Deletion of 11q (including MLL) is classified as a very good cytogenetic prognostic indicator in MDS.
- Deletion of 5q- and 20q- are classified as good cytogenetic prognostic indicators in MDS.
- 7q- and +8 are classified as intermediate cytogenetic prognostic indicators in MDS.
- -7 is classified as a poor cytogenetic prognostic indicator in MDS.
03/26/2021