T & B cell screen

Alphabetical Test listing

T & B cell screen-12420

  
T & B cell screen
  
12420
  
LAB12420
  
T&B
  
Flow
Flow Cytometry
B cell
Full B panel
Leukemia
Lymphoma
Lymphocyte phenotype
Lymphocyte screen
T cell
  
Tubes:
B1
T1

Markers:
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD19
CD20
CD45
CD56
Kappa
Lambda
TCRγδ (TCR gamma delta)
TRBC1
  

Screen for aberrant T cells, light chain-restricted B cells and excess NK cells

  

Pathology orders only

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

  

Lavender (EDTA), 4mL

  
Body fluid
Bone marrow
Cerebrospinal fluid (CSF)
Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Include previous Allina case number, if applicable.

  

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Cerebrospinal fluid (CSF)

Refrigerated

Tissue

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B+ fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

  

Tubes and Markers:

Tubes: B1 and T1

Markers: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, CD45, CD56, Kappa, Lambda, TCRγδ (TCR gamma delta) and TRBC1

  
88184
88185 x 14
  
08/05/2021
  
02/13/2024
  
01/09/2024