Alpha defensin, lateral flow assay, synovial fluid

Alphabetical Test listing

Alpha defensin, lateral flow assay, synovial fluid-16029

For specimens not obtained from a prosthetic joint, order MSO (LAB994) Joint Infection Panel, PCR, Synovial Fluid

Test name:

Alpha defensin, lateral flow assay, synovial fluid

Test number:

16029

Excellian order number:

LAB16029

Abbreviation:

ALDEF

Alternate names:

ALDEF
Alpha defensin
Alpha-defensin
PJI
Prosthetic joint infection
Synovasure

Useful for:

Detection of alpha defensins 1-3, human host response proteins, in synovial fluid of adults with a total joint replacement who are being evaluated for revision surgery.

Limitations:

This test is not intended to be used to determine timing for reimplantation in 2-stage procedures
This test should be used for patients with a total joint prosthesis only

Specimen type:

Synovial fluid

Collection container:

Red serum vial/tube, 5 mL

Volume:

0.6 mL

Minimum volume:

0.1 mL

Processing instructions:

Transfer fluid from the syringe used for collection to a Red serum vial/tube, 5 mL

Transport container:

Red serum vial/tube, 5 mL

Alternate collection container:

Screw cap transfer vial/tube (Mayo T914)

Alternate transport container:

Screw cap transfer vial/tube (Mayo T914)

Required forms/information:

Specimen source
Type of joint

Transport and stability:

Refrigerated (strict) – 7 days

Ambient - 7 days

Frozen - NO

Specimen retention time:

7 days

Reason for rejection:

Gross hemolysis
If fluid is not obtained from a prosthetic joint, testing will be canceled

Performing lab:

Mayo Clinic Laboratories (ALDEF): R-MM

Days set up:

Mo - Fr, Su

TAT:

2 days

Method:

Lateral Flow Assay (LFA)

Reference ranges:

Negative; No alpha defensin detected in synovial fluid, suggesting absence of prosthetic joint infection.

CPT codes:

83518

Date created:

10/16/2020

Revised date:

02/25/2026

Review date:

02/25/2026