HMB 45 by IHC

Alphabetical Test listing

HMB 45 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

HMB 45 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Human melanoma black

Tests included:

All IHC stains will include a positive control tissue

Useful for:

HMB45 reacts with most melanomas (approximately 80%) as well as with benign melanocytes such as in junctional melanocytes, Spitz nevi, and cellular blue nevi
HMB45 is usually negative in desmoplastic and spindle cell melanomas
HMB45 usually is nonreactive with normal melanocytes in normal adult skin, intradermal nevi, and melanophages
For melanomas, order both S-100 and HMB45. If lesion is heavily pigmented, consider specifying the alkaline phosphatase (red) staining method
No known reactivity with other carcinomas (false reports in literature regarding HMB45 positive breast carcinomas¹)
Unique reaction of HMB45 in pulmonary lymphangiomyomatosis² and angiomyolipomas³

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded tissue

Transport container:

Formalin-fixed, paraffin-embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications:

HMB45 recognizes a premelanosome glycoprotein

Staining pattern:

Cytoplasmic based (may show focal/mosaic pattern in melanomas)

References

Bonetti et. al.: False-Positive Immunostaining of Normal Epithelial and Carcinomas with Ascites Fluid Preparations of Antimelanoma Monoclonal Antibody HMB-45; American Journal of Clinical Pathology; 95:454-459, 1991.
Bonetti, et. al.: Transbronchial Biopsy in Lymphangiomyomatosis; American Journal of Surgical Pathology; 17(11):1092-1102, 1993. Sturtz, et. al.: Angiomyolipomas: The Nature and Expression of the HMB45 Antigen; Modern Pathology; 7(8):842-845, 1994.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

07/03/2017

Revised date:

10/19/2018

Review date:

01/12/2024