Adrenocorticotropin by IHC

Alphabetical Test listing

Adrenocorticotropin by IHC-12376 - Technical only, 12379 - Technical & interpretation

Adrenocorticotropin by IHC
12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue
  • Immunoperoxidase staining for the presence of pituitary hormones helps distinguish pituitary adenomas and the rare primary pituitary carcinomas from other tumors occurring in the region of the sella (such as craniopharyngioma, metastatic carcinoma, meningioma, lymphoma, leukemia and plasmacytoma), none of which contain or produce pituitary hormones. Positive staining for specific pituitary hormones can divide the pituitary adenomas into subclasses based on the corresponding cell type present
  • Negative staining for pituitary hormones does not rule out a pituitary adenoma since some contain none of the known adenohypophyseal hormones
  • ACTH cell adenomas comprise 10-15% of all pituitary adenomas, including the endocrinologically silent corticotrophic adenomas (2%) unassociated with biochemical or clinical evidence of cortisol excess

Submit a formalin-fixed, paraffin embedded (FFPE) tissue block


FFPE tissue block


Tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • This antibody reacts with ACTH. It recognizes the corticotrophic cells of the pituitary gland. In normal pituitary gland, these are mostly contained in the central portion of the adenohypophysis and constitute 15-20% of the cells in the gland

 Staining patterns

  • Cytoplasmic based staining

88342 - 1st stain
88341 - each additional stain