Plasma cell monitoring

Alphabetical Test listing

Plasma cell monitoring-12420

Test name:

Test number:

12420

Excellian order number:

LAB12420

Abbreviation:

PATR

Alternate names:

Flow
Flow cytometry
Immunophenotyping
MGUS
MM
Monoclonal gammopathy, plasma cell
Multiple Myeloma
Multiple Myeloma monitoring
P2
Plasma cell Immunophenotyping
Plasma cell follow-up

Tests included:

CD19
CD20
CD38
CD45
CD56
IRF4 (nuclear)
Kappa (cytoplasmic)
Lambda(cytoplasmic)
VS38(cytoplasmic)

Useful for:

Detecting residual disease in treated multiple myeloma patients at follow-up

Limitations:

Pathology orders only

The P2 assay is a high-sensitivity assay validated to a sensitivity of 0.01% (1 abnormal plasma cell/10,000 normal cells). This assay is NOT a minimal/measureable residual (MRD) assay, which requires a validated sensitivity of 0.001% (1/100,000). For follow-up of patients who are negative for residual abnormal plasma cells in the P2 assay, we recommend EDTA-anticoagulated bone marrow aspirate be submitted to the Mayo Clinic Laboratories for formal MRD testing (Mayo Clinic Laboratories test code: MRDMM).

Specimen type:

Bone marrow

Collection container:

Heparinized syringe

Volume:

3.0 mL

Minimum volume:

1.0 mL

Collection instructions:

Collect 3.0 mL (minimum 1.0mL) bone marrow aspirate in a sodium heparinized syringe and transfer to an anticoagulant free tube.

Bone marrow specimen transport kit

Transport container:

Bone marrow transport vial/tube, 8mL or

Falcon vial/tube - 15mL

Alternate specimen type:

Bone marrow core

Alternate collection container:

RPMI with Hepes/Pen strep/FBS -15 mL

Alternate collection instructions:

If you do not use a heparinized syringe, collect 3.0 mL (minimum 1.0mL) bone marrow aspirate and transfer to a Dk green Sodium heparin (Na hep), no gel (preferred) or a Lavender (EDTA), 4mL (acceptable)
Mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Alternate transport container:

Bone marrow aspirate:

If you do not use a heparinized syringe to collect the sample:

Dk green Sodium heparin (Na hep), no gel (preferred)

Lavender (EDTA), 4mL (acceptable)

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Bone marrow core:

RPMI with Hepes/Pen strep/FBS -15 mL

Required forms/information:

Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the Flow cytometry requisition

Transport and stability:

Ambient (preferred) - 72 hours

Refrigerated (discouraged)

Frozen - NO

Specimen retention time:

14 days

Reason for rejection:

Improper labels (unlabeled or mislabeled)
Improper anticoagulant or ratio
Delay in transport
Improper storage temperature affecting results
Improper container
Leaking container resulting in compromised specimen
Quantity not sufficient (QNS)

Performing lab:

AHL - Flow Cytometry: F

Days set up:

Mo - Fr

TAT:

2 days

Method:

Flow cytometry

Clinical information:

Tubes:

Markers:

CD19, CD20, CD38, CD45, CD56, IRF4 (nuclear), Kappa (cytoplasmic), Lambda (cytoplasmic) and VS38 (cytoplasmic).

Cautions:

CPT codes:

88184
88185 × 8*
*Charges may vary based on immunophenotype of cells in the specimen

Hospital EAP/charge code:

88184 - 17021303
88185 (each) - 17021304

Clinic EAP/charge code:

88184 - 30881840
88185 (each) - 308818500

Date created:

06/03/2021

Review date:

01/09/2024