Vimentin by IHC

Alphabetical Test listing

Vimentin by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Vimentin by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Vim IHC
  
  • All IHC stains will include a positive control tissue
  
  • Use for internal control to gauge general preservation of antigens in tissue (particularly useful in blocks processed at outside hospitals).1
  • Use to identify mesenchymal cells (lymphocytes are not consistently stained; some glial cells are positive)
  • Vim may also be expressed in rapidly dividing epithelial cells
  • Many carcinomas are CK and Vim positive:
    • Breast
    • Choroid plexus
    • Endometrium
    • Kidney
    • Lung
    • Ovary
    • Prostate
    • Salivary gland
    • Stomach
    • Thyroid
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

Submit a formalin-fixed, paraffin embedded tissue block

  

Ambient (preferred) 

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

Specifications

  • Reacts with 57-KD intermediate filament vimentin, found in a variety ofmesenchymal cells including fibroblasts, endothelial cells, melanocytes, lymphoid cells, and macrophages

Staining pattern

  • Cytoplasmic based staining

References

  1. Battifore, H: Assessment of Antigen Damage in Immunohistochemistry: The Vimentin Internal Control; American Journal of Clinical Pathol 96:669-671, 1991.
  2. Azumi, et. al.: The Distribution of Vimentin and Keratin in Epithelial and Non-epithelial Neoplasms: A Comprehensive Immunohistochemical Study on Formalin- and Alcohol-Fixed Tumors; American Journal of Clinical Pathol 88:286-296, 1987
  
88342 - 1st stain
88341 - each additional stain
  
09/13/2017
  
10/17/2018
  
10/02/2020