Serotonin release assay (SRA)

Alphabetical Test listing

Serotonin release assay (SRA)-994

 

 

  
Serotonin release assay (SRA)
  
994
  
LAB994
  
MSO
  
Functional HIT assay
Heparin-dependent platelet antibody
  
SRA, Low Dose Heparin
SRA, High Dose Heparin
SRA, Interpretation
  

Confirmatory assay used in the evaluation of heparin-induced thrombocytopenia (HIT).

  
Serum
  
  
1.0 mL
  
0.5 mL
Submission of the minimum volume does not allow for repeat testing
  

Immediately following collection, thoroughly mix sample by gently inverting 5 times

  
  1. Allow sample to clot for a minimum of 30 minutes
  2. Spin within two (2) hours of sample collection
  3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
  4. Freeze
  
  
  1. Allow sample to clot
  2. Spin 
  3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum, within two (2) hours of sample collection
  4. Freeze
  

Frozen

  

Room temperature specimen received; grossly hemolyzed, icteric, and lipemic samples

  
LabCorp Burlington (150018): R-NX
  
Mo - Fr
  
8 days
  

The SRA employs washed donor platelets and detects their activation by measuring the release of endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by a highly sensitive liquid chromatography/ tandem mass spectroscopy method. Therapeutic levels of heparin included in the assay mix allow for the formation of PF4/heparin complexes on the donor platelet surface to provide an antigen target for the HIT antibodies. The specificity of the assay is increased by assessing the inhibition of platelet activation by adding heparin in excess, thus proving the heparin dependency of the serotonin release.

  

An interpretive report will be provided

  
82542
  
SRA, Low Dose Heparin 77728-4
SRA High Dose Heparin 50727-7
  
05/29/2019
  
05/30/2023
  
05/30/2023