Serotonin release assay (SRA)

Alphabetical Test listing

Serotonin release assay (SRA)-994

  
Serotonin release assay (SRA)
  
994
  
LAB994
  
MSO
  
Functional HIT assay
Heparin-dependent platelet antibody
  

Confirmatory assay used in the evaluation of heparin-induced thrombocytopenia (HIT).

  
Serum
  
  
1.0 mL
  
0.5 mL
Note: This volume does not allow for repeat testing
  

Spin, separate and freeze.

  
  

Frozen

  

Room temperature specimen received; grossly hemolyzed, icteric, and lipemic samples

  
LabCorp Burlington (150018): R-NX
  
Mo - Fr
  
2 - 4 days
  

The SRA employs washed donor platelets and detects their activation by measuring the release of endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by a highly sensitive liquid chromatography/ tandem mass spectroscopy method. Therapeutic levels of heparin included in the assay mix allow for the formation of PF4/heparin complexes on the donor platelet surface to provide an antigen target for the HIT antibodies. The specificity of the assay is increased by assessing the inhibition of platelet activation by adding heparin in excess, thus proving the heparin dependency of the serotonin release.

  

An interpretive report will be provided

  
82542
  
Result 77728-4
  
05/29/2019
  
12/18/2019